Chromatin reorganization as a major factor in the control of nuclear stiffness

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Anzahl der Dateien: 31
macros.zip (19.38KB) 
Fig1.zip (Figure 1: nuclear stiffness is independent of the cytosolic components) (171.1MB) 
Fig2.zip (Figure 2: MeCP2 clustering of heterochromatin directly increases nuclear stiffness) (137.6MB) 
Fig2.z01 ((needs to be downloaded together with Fig2 to properly unzip)) (4GB) 
Fig3.zip (Figure 3: MeCP2 has a main role in increasing nuclear stiffness in neural differentiation) (895.2MB) 
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Beschreibung

DNA organization on chromatin has been largely studied for its consequences in gene expression, but its contribution to the cell mechanics is often neglected despite the nucleus being the stiffest organelle in the cell. Here, we show that MeCP2 directly increases the nuclear stiffness in a similar fashion as it clusters the heterochromatin. Moreover, we show how this phenomenon occurs during cellular differentiation and how it can be disrupted by mutations related to diseases.

Schlagwort

MeCP2;mechanobiology;stiffness;atomic force microscope;Rett syndrome;chromatin organization;neuronal differentiation;myogenic differentiation

DFG-Fächer

2.11-02 Biophysik
2.11-03 Zellbiologie

URI

Zugehörige Drittmittelprojekte

DFG | CA198/16-1 | Rölle und Regulation
DFG | CA198/19-1 | Wie die Zusammensetz

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Creative Commons Attribution-NonCommercial 4.0
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