MeCP2-driven chromatin organization controls nuclear stiffness
| dc.contributor.author | Romero, Hector | |
| dc.contributor.author | Amiri, Anahid | |
| dc.contributor.author | Pabba, Maruthi Kumar | |
| dc.contributor.author | Zhang, Hui | |
| dc.contributor.author | Berg, Veronika | |
| dc.contributor.author | Arroyo, Maria | |
| dc.contributor.author | Prorok, Paulina | |
| dc.contributor.author | Zhadan, Andreas | |
| dc.contributor.author | Marah Mahmoud | |
| dc.contributor.author | Trautwein, Nina | |
| dc.contributor.author | Laube, Bodo | |
| dc.contributor.author | Dietz, Christian | |
| dc.contributor.author | Stark, Robert W. | |
| dc.contributor.author | Cardoso, M. Cristina | |
| dc.date.accessioned | 2024-11-13T08:30:15Z | |
| dc.date.available | 2024-11-13T08:30:15Z | |
| dc.date.created | 2024 | |
| dc.date.issued | 2024-11-13 | |
| dc.description | DNA organization on chromatin has been largely studied for its consequences in gene expression, but its contribution to the cell mechanics is often neglected despite the nucleus being the stiffest organelle in the cell. Here, we show that MeCP2 directly increases the nuclear stiffness in a similar fashion as it clusters the heterochromatin. Moreover, we show how this phenomenon occurs during cellular differentiation and how it can be disrupted by mutations related to diseases. | de_DE |
| dc.identifier.uri | https://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4381 | |
| dc.identifier.uri | https://doi.org/10.48328/tudatalib-1590 | |
| dc.language.iso | en | de_DE |
| dc.rights.license | CC-BY-NC-4.0 (https://creativecommons.org/licenses/by-nc/4.0) | |
| dc.subject | MeCP2 | de_DE |
| dc.subject | mechanobiology | de_DE |
| dc.subject | stiffness | de_DE |
| dc.subject | atomic force microscopy | de_DE |
| dc.subject | Rett syndrome | de_DE |
| dc.subject | chromatin organization | de_DE |
| dc.subject | neuronal differentiation | de_DE |
| dc.subject.classification | 2.11-02 | |
| dc.subject.classification | 2.11-03 | |
| dc.subject.ddc | 570 | |
| dc.title | MeCP2-driven chromatin organization controls nuclear stiffness | de_DE |
| dc.type | Dataset | de_DE |
| dcterms.accessRights | openAccess | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| tuda.project | DFG | CA198/16-1 | Rölle und Regulation | |
| tuda.project | DFG | CA198/19-1 | Wie die Zusammensetz | |
| tuda.unit | TUDa |
Files
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| Name | Description | Size | Format | |
|---|---|---|---|---|
| macros.zip | Self-made FIJI (Image J) and Matlab macros used in this paper | 26.01 KB | ZIP-Archivdateien | |
| Fig1.zip | Figure 1. Nuclear stiffness does not depend on the cytosolic components. | 442.04 MB | ZIP-Archivdateien | |
| Fig2.zip | Figure 2. Heterochromatin clustering driven by MeCP2 increases nuclear stiffness. | 788.52 MB | ZIP-Archivdateien | |
| Fig2.z01 | (needs to be download together with Figure 2 ) | 4 GB | Unknown data format | |
| Fig3.zip | Figure 3. Deletion of MeCP2 severely impairs the increase of stiffness during neuronal differentiation. | 801.57 MB | ZIP-Archivdateien | |
| Fig3.z01 | (needs to be downloaded with Fig_3.zip) | 4 GB | Unknown data format | |
| Fig4.zip | Figure 4. Rett syndrome mutations of MeCP2 impair the increase of nuclear stiffness. | 44.21 MB | ZIP-Archivdateien | |
| Fig4.z01 | (Needs to be downloaded with Fig_4.zip) | 4 GB | Unknown data format | |
| Fig5.zip | Figure 5. MeCP2 regulates the expression of the mechanotransduction-related genes Notch2 and Tgfbr1. | 51.22 MB | ZIP-Archivdateien | |
| FigS2.zip | Generation and characterization of J1 MeCP2 KO embryonic stem cells | 3.12 GB | ZIP-Archivdateien | |
| FigS2.z01 | (needs to be downloaded with FigS2.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.zip | Generation and characterization of J1 NSC wt and J1 NSC MeCP2 KO | 2.83 GB | ZIP-Archivdateien | |
| FigS4.z01 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z02 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z03 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z04 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z05 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z06 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z07 | (needs to be downloaded with FigS4.zip to properly unzip it) | 4 GB | Unknown data format | |
| FigS4.z08 | 4 GB | Unknown data format |