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Chromatin reorganization as a major factor in the control of nuclear stiffness

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macros.zip (19.38 KB)
 Fig1.zip (Figure 1: nuclear stiffness is independent of the cytosolic components) (171.17 MB)
 Fig2.zip (Figure 2: MeCP2 clustering of heterochromatin directly increases nuclear stiffness) (137.63 MB)
 Fig2.z01 ((needs to be downloaded together with Fig2 to properly unzip)) (4 GB)
 Fig3.zip (Figure 3: MeCP2 has a main role in increasing nuclear stiffness in neural differentiation) (895.28 MB)

Date

2024-11-13

Type

Dataset

Authors

Romero, Hector
Amiri, Anahid
Pabba, Maruthi Kumar
Zhang, Hui
Berg, Veronika
Arroyo, Maria
Prorok, Paulina
Trautwein, Nina
Laube, Bodo
Dietz, Christian
Stark, Robert W.
Cardoso, M. Cristina

Journal Title

Journal ISSN

Volume Title

Publisher

Abstract

Description

DNA organization on chromatin has been largely studied for its consequences in gene expression, but its contribution to the cell mechanics is often neglected despite the nucleus being the stiffest organelle in the cell. Here, we show that MeCP2 directly increases the nuclear stiffness in a similar fashion as it clusters the heterochromatin. Moreover, we show how this phenomenon occurs during cellular differentiation and how it can be disrupted by mutations related to diseases.

Keywords

MeCP2, mechanobiology, stiffness, atomic force microscope, Rett syndrome, chromatin organization, neuronal differentiation, myogenic differentiation

Citation

Identifier

https://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4381
 https://doi.org/10.48328/tudatalib-1590

Endorsement

DFG Classification

2.11-02 Biophysik
2.11-03 Zellbiologie

Project(s)

DFG | CA198/16-1 | Rölle und Regulation
DFG | CA198/19-1 | Wie die Zusammensetz

Faculty

Collections

License

CC-BY-NC 4.0 - Attribution-NonCommercial 4.0 International
Except where otherwise noted, this license is described as CC-BY-NC 4.0 - Attribution-NonCommercial 4.0 International