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Chromatin reorganization as a major factor in the control of nuclear stiffness

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macros.zip (Self-made FIJI (Image J) and Matlab macros used in this paper) (26.01 KB)
 Fig1.zip (Figure 1. Nuclear stiffness does not depend on the cytosolic components.) (442.04 MB)
 Fig2.zip (Figure 2. Heterochromatin clustering driven by MeCP2 increases nuclear stiffness.) (788.52 MB)
 Fig2.z01 ((needs to be download together with Figure 2 )) (4 GB)
 Fig3.zip (Figure 3. Deletion of MeCP2 severely impairs the increase of stiffness during neuronal differentiation.) (801.57 MB)

Date

2024-11-13

Type

Dataset

Authors

Romero, Hector
Amiri, Anahid
Pabba, Maruthi Kumar
Zhang, Hui
Berg, Veronika
Arroyo, Maria
Prorok, Paulina
Zhadan, Andreas
Marah Mahmoud
Trautwein, Nina
Laube, Bodo
Dietz, Christian
Stark, Robert W.
Cardoso, M. Cristina

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Abstract

Description

DNA organization on chromatin has been largely studied for its consequences in gene expression, but its contribution to the cell mechanics is often neglected despite the nucleus being the stiffest organelle in the cell. Here, we show that MeCP2 directly increases the nuclear stiffness in a similar fashion as it clusters the heterochromatin. Moreover, we show how this phenomenon occurs during cellular differentiation and how it can be disrupted by mutations related to diseases.

Keywords

MeCP2, mechanobiology, stiffness, atomic force microscopy, Rett syndrome, chromatin organization, neuronal differentiation

Citation

Identifier

https://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4381
 https://doi.org/10.48328/tudatalib-1590

Endorsement

DFG Classification

2.11-02 Biophysik
2.11-03 Zellbiologie

Project(s)

DFG | CA198/16-1 | Rölle und Regulation
DFG | CA198/19-1 | Wie die Zusammensetz

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CC-BY-NC 4.0 - Attribution-NonCommercial 4.0 International
Except where otherwise noted, this license is described as CC-BY-NC 4.0 - Attribution-NonCommercial 4.0 International