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dc.contributor.authorHastert, Florian D.
dc.contributor.authorWeber, Jasmin
dc.contributor.authorBauer, Christina
dc.contributor.authorZhadan, Andreas
dc.contributor.authorSingh, Deepanshu N.D.
dc.contributor.authorDix, Thomas C.
dc.contributor.authorArnold, Roland
dc.contributor.authorBessonov, Sergey
dc.contributor.authorSoller, Matthias
dc.contributor.authorLeonhardt, Heinrich
dc.contributor.authorCardoso, M. Cristina
dc.contributor.authorArroyo, Maria
dc.date.accessioned2025-02-01T18:37:18Z
dc.date.available2025-02-01T18:37:18Z
dc.date.issued2024-12
dc.identifier.urihttps://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4453
dc.descriptionThe dynamic regulation of RNA metabolism plays a crucial role in cellular function, with emerging evidence suggesting a role for RNA modifications in this process. This study explores the relationship between RNA splicing and the TET dioxygenase activity, shedding light on the role of hm5C (RNA 5-hydroxymethylcytosine), and TET proteins, in RNA metabolism. Integrating data from mass spectrometry, AlphaFold structural modeling, microscopic analysis, and different functional assays including in vitro splicing, TET proteins were found to regulate splicing. We show that TET1, TET2, and TET3 interact with the splicing factors U2AF1 and U2AF2. Interestingly, TET dioxygenases localize in splicing speckles in mammalian and Drosophila cells. TET speckles association is RNA dependent, as it is TET interaction with splicing factors. Furthermore, in vitro splicing assays revealed that all three TET proteins promote splicing efficiency, and the oxidation of m5C to hm5C can restore splicing efficiency in vitro. The latter highlights the regulatory role of cytosine modifications in RNA metabolism. These findings provide insights into the complex interplay between RNA modifications and splicing, suggesting a multifaceted role for Tet proteins in RNA metabolism beyond its canonical DNA demethylation function.de_DE
dc.language.isoende_DE
dc.rightsCreative Commons Attribution-NonCommercial 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectRNA modificationsde_DE
dc.subjectsplicingde_DE
dc.subjectTET dioxygenasesde_DE
dc.subjectU2AFde_DE
dc.subjectEpitranscriptomicsde_DE
dc.subject5-hydroymethylcytosinede_DE
dc.subject.classification2.11-03 Zellbiologiede_DE
dc.subject.ddc570
dc.titleTET dioxygenases localize at splicing speckles and promote RNA splicingde_DE
dc.typeDatasetde_DE
dc.typeTextde_DE
dc.typeImagede_DE
dc.typeModelde_DE
dc.description.versionFor reviewde_DE
tud.unitTUDa


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Creative Commons Attribution-NonCommercial 4.0
Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: Creative Commons Attribution-NonCommercial 4.0