TUdatalib : Uptake of substances into living mammalian cells by microwave induced perturbation of the plasma membrane

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dc.contributor.author	Manuela, Milden-Appel
dc.contributor.author	Markus, Paravicini
dc.contributor.author	Jannick P., Milden
dc.contributor.author	Martin, Schüßler
dc.contributor.author	Rolf, Jakoby
dc.contributor.author	M. Cristina, Cardoso
dc.date.accessioned	2024-03-25T10:42:06Z
dc.date.available	2024-03-25T10:42:06Z
dc.date.issued	2024-03-22
dc.identifier.uri	https://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4191
dc.identifier.uri	https://doi.org/10.48328/tudatalib-1397
dc.description	Delivering foreign molecules and genetic material into cells is a crucial process in life sciences and biotechnology, resulting in great interest in effective cell transfection methods. Importantly, physical transfection methods allow delivery of molecules of different chemical composition and are, thus, very flexible. Here, we investigated the influence of microwave radiation on the transfection and survival of mammalian cells. We made use of an optimized microwave-poration device and analyzed its performance (frequency and electric field strength) in comparison with simulations. We, then, tested the effect of microwave irradiation on cells and found that 18 GHz had the least impact on cell survival, viability, cell division and genotoxicity while 10 GHz drastically impacted cell physiology. Using live-cell fluorescence microscopy and image analysis, we tested the uptake of small chemical substances, which was most efficient at 18 GHz and correlated with electric field strength and frequency. Finally, we were able to obtain cellular uptake of molecules of molecules of very different chemical composition and sizes up to whole immunoglobulin antibodies. In conclusion, microwave-induced poration enables the uptake of widely different substances directly into mammalian cells growing as adherent cultures and with low physiological impact.	de_DE
dc.language.iso	en	de_DE
dc.rights	Creative Commons Attribution-NonCommercial 4.0
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/
dc.subject.classification	2.11-03 Zellbiologie	de_DE
dc.subject.ddc	570
dc.title	Uptake of substances into living mammalian cells by microwave induced perturbation of the plasma membrane	de_DE
dc.type	Dataset	de_DE
dc.type	Image	de_DE
tud.project	DFG \| CA198/17-1 \| Aufnahme in Zellen d	de_DE
tud.project	DFG \| JA921/73-1 \| Aufnahme in Zellen d	de_DE
tud.unit	TUDa
tud.history.classification	Version=2020-2024;201-03 Zellbiologie