TUdatalib : Isotachophoresis with oscillating sample zones

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dc.contributor.author	Gebhard, Florian
dc.contributor.author	Bonart, Henning
dc.contributor.author	Roy, Tamal
dc.contributor.author	Meckel, Tobias
dc.contributor.author	Hardt, Steffen
dc.date.accessioned	2024-03-03T18:15:12Z
dc.date.available	2024-03-03T18:15:12Z
dc.date.issued	2024-02-08
dc.identifier.uri	https://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4150
dc.identifier.uri	https://doi.org/10.48328/tudatalib-1359
dc.description	Microfluidic isotachophoresis (ITP) is an electrophoretic technique that can be used to co-focus reactants and thereby accelerate chemical reactions. The fluorescence images provided here (fluorescenceImages1.zip to fluorescenceImages3.zip) show the isotachophoretic transport of Alexa fluor 647 carboxylic acid (AF647) and Fluorescein using an oscillating electric field with a DC offset. The two fluorophores are focused in sample zones separated by a spacer. Different amplitudes, frequencies and offsets were applied with a HVS448 6000 D high voltage sequencer (Labsmith). The fluorescence images were obtained with a Nikon Eclipse Ti microscope and an Andor iXon+ 897 EMCCD camera. A 10x Plan Fluor objective (NA = 0.3, Nikon) and a Intensilight C-HGFI mercury fiber illuminator (Nikon) were used. The exposure time was set to 23 ms and the frame rate to 40 fps. More information on the experimental procedure can be found in the paper that will be linked below. The folder contains a description of the amplitude, frequency, DC offset, and spacer concentration that correspond to each image file. The code (code.zip) was used to quantify the spatial overlap of the sample zones separated by 500 µM of spacer.	de_DE
dc.language.iso	en	de_DE
dc.relation	IsSupplementTo;DOI;https://doi.org/10.1021/acs.analchem.3c04606
dc.rights	Creative Commons Attribution 4.0
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	isotachophoresis	de_DE
dc.subject	fluorescence images	de_DE
dc.subject	fluorescence microscopy	de_DE
dc.subject	oscillating electric field	de_DE
dc.subject	reaction control	de_DE
dc.subject	control of spatial overlap	de_DE
dc.subject.classification	324-01 Analytische Chemie	de_DE
dc.subject.classification	404-03 Strömungsmechanik	de_DE
dc.subject.ddc	540
dc.subject.ddc	620
dc.title	Isotachophoresis with oscillating sample zones	de_DE
dc.type	Software	de_DE
dc.type	Image	de_DE
tud.unit	TUDa