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dc.contributor.authorPradhan, Sunil Kumar
dc.contributor.authorLozoya, Teresa
dc.contributor.authorProrok, Paulina
dc.contributor.authorYuan, Yue
dc.contributor.authorLehmkuhl, Anne
dc.contributor.authorZhang, Peng
dc.contributor.authorCardoso, M. Cristina
dc.date.accessioned2024-03-12T11:38:44Z
dc.date.available2024-01-24T19:48:24Z
dc.date.available2024-01-25T15:34:03Z
dc.date.available2024-03-12T11:38:44Z
dc.date.issued2023
dc.identifier.urihttps://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4110.3
dc.descriptionDNA replication is a fundamental process ensuring the complete and accurate maintenance of the genome each time cells divide. This is particularly relevant early in development when cells divide profusely, later giving rise to entire organs. Increasing number of studies analyze DNA replication progression genome-wide but lack 3D spatial information as well as have a low temporal resolution. Importantly, there is little information regarding repeat elements which constitute more than half of the human genome. Here, we analyze and compare the genome replication progression in human stem cells, pluripotent stem cells and differentiated somatic cells. Using single-cell microscopic approaches and quantitative image analysis, we map the spatio-temporal genome replication of chromatin as a function of its compaction level. Furthermore, we mapped the replication timing of subchromosomal tandem repeat regions as well as the interspersed repeat sequences elements. Here, we found developmental changes in the replication timing of rDNA repeats. Finally, we compared the single-cell microscopic data with data from genome-wide sequencing approaches.de_DE
dc.language.isoende_DE
dc.rightsCreative Commons Attribution-NonCommercial 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectinduced pluripotent stem cellsde_DE
dc.subjectgenome replication progressionde_DE
dc.subjectrepli-FISHde_DE
dc.subjectchromatin compactionsde_DE
dc.subject.classification201-03 Zellbiologiede_DE
dc.subject.ddc570
dc.titleDevelopmental changes in genome replication progression in pluripotent versus differentiated human cellsde_DE
dc.typeDatasetde_DE
dc.typeTextde_DE
dc.typeSoftwarede_DE
dc.typeImagede_DE
dc.typeAudiovisualde_DE
dc.typeWorkflowde_DE
dc.description.versionFor reviewde_DE
tud.unitTUDa


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Creative Commons Attribution-NonCommercial 4.0
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