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dc.contributor.authorGebhard, Florian
dc.contributor.authorHartmann, Johannes
dc.contributor.authorHardt, Steffen
dc.date.accessioned2023-02-07T12:40:59Z
dc.date.available2023-02-07T12:40:59Z
dc.date.issued2020
dc.identifier.urihttps://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/3733
dc.identifier.urihttps://doi.org/10.48328/tudatalib-1078
dc.descriptionBased on fluorescence microscopy, the electrophoretic transfer of proteins across the liquid–liquid interface in microfluidic aqueous two-phase systems (ATPS) was investigated. Specifically, the behavior of bovine serum albumine (BSA) and bovine γ-globulin (BγG) in an ATPS containing polyethylene glycol and dextran was observed. Both proteins, initially dissolved in the dextran phase, accumulated significantly close to the three-phase contact lines between the two aqueous phases and the solid surface upon application of an electric field. The accumulation process is superposed by crossing of the proteins into the PEG phase, which also occurs mainly at the three-phase contact line. Differences in their accumulation/transfer behavior allow a separation of the two proteins. In this collection, we present the fluorescence images of the accumulation and transfer dynamics as observed with widefield and confocal microscopy. The matlab files contain the code that was used to evaluate the widefield images of protein accumulation at the phase boundary.de_DE
dc.language.isoende_DE
dc.relationIsSupplementTo;DOI;https://doi.org/10.1039/D0SM01921F
dc.rightsCreative Commons Attribution 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectATPSde_DE
dc.subjectfluorescence imagesde_DE
dc.subjectfluorescence microscopyde_DE
dc.subjectconfocal microscopyde_DE
dc.subjectimage processingde_DE
dc.subjectprotein separationde_DE
dc.subject.classification323-01 Physikalische Chemie von Molekülen, Flüssigkeiten und Grenzflächen, Biophysikalische Chemiede_DE
dc.subject.classification324-01 Analytische Chemiede_DE
dc.subject.classification404-03 Strömungsmechanikde_DE
dc.subject.ddc620
dc.subject.ddc540
dc.titleAccumulation and separation of bovine serum albumin and bovine γ-globulin at liquid/liquid interfaces of aqueous two-phase systemsde_DE
dc.typeSoftwarede_DE
dc.typeImagede_DE
tud.unitTUDa


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Creative Commons Attribution 4.0
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