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Microfluidic isotachophoresis in constant-current mode with Alexa Fluor 647 carboxylic acid

dc.contributor.authorBonart, Henning
dc.contributor.authorGebhard, Florian
dc.contributor.authorHecht, Lukas
dc.contributor.authorRoy, Tamal
dc.contributor.authorLiebchen, Benno
dc.contributor.authorHardt, Steffen
dc.date.accessioned2022-07-11T07:04:45Z
dc.date.available2022-07-11T07:04:45Z
dc.date.issued2022-07-11
dc.identifier.urihttps://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/3529
dc.identifier.urihttps://doi.org/10.48328/tudatalib-914
dc.descriptionMicrofluidic isotachophoresis (ITP) is an electrophoretic technique that can be used to concentrate analytes, thereby facilitating and accelerating their detection. The fluorescence images show the isotachophoretic transport of Alexa fluor 647 carboxylic acid (AF 647) with a constant current of 5 µA. Constant-current ITP has the main effect that the velocity at which the sample migrates through the microchannel is constant over time. By choosing sample concentrations between 0 and 10 ng/L, the signal-to-noise ratio is varied. Only at higher concentrations can the sample be detected without further image processing. The data were obtained to test the capability of a newly developed image processing method that allows the detection of samples even at low concentrations without any additional instrumentation effort. The fluorescence images were obtained with a Nikon Eclipse Ti microscope and an Andor iXon+ 897 EMCCD camera. A 10x S-Fluor objective (NA = 0.5, Nikon) and a 639 nm laser were used. The exposure time was set to 20 ms and the frame rate to 46 fps. More information on the experimental procedure can be found in the paper that will be linked below.de_DE
dc.language.isoende_DE
dc.rightsCreative Commons Attribution 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectfluorescence imagesde_DE
dc.subjectisotachophoresisde_DE
dc.subjectfluorescence microscopyde_DE
dc.subjectpeak-mode ITPde_DE
dc.subjectconstant-current ITPde_DE
dc.subjectsample detectionde_DE
dc.subject.classification3.14-01 Analytische Chemiede_DE
dc.subject.classification4.22-03 Strömungsmechanikde_DE
dc.subject.ddc540
dc.subject.ddc620
dc.titleMicrofluidic isotachophoresis in constant-current mode with Alexa Fluor 647 carboxylic acidde_DE
dc.typeImagede_DE
dc.description.version1.de_DE
tud.unitTUDa
tud.history.classificationVersion=2020-2024;324-01 Analytische Chemie
tud.history.classificationVersion=2020-2024;404-03 Strömungsmechanik


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