TUdatalib Upgrade

Am 2. Juni erfolgte ein TUdatalib Upgrade auf eine neue Softwareversion. Dieses Upgrade bringt wichtige Neuerungen mit sich. Eine Übersicht finden Sie in der Dokumentation
On June 2nd, TUdatalib was upgraded to a new software version. This upgrade introduced major changes to the system. Please see our documentation for an overview.

 

Superresolution-Compatible DNA Labeling Technique with Silicon Rhodamine -Linked Nucleotide Reveals Chromatin Mobility and Organization Changes During Neuronal Differentiation

Simple item page
dc.contributor.author Pabba, Maruthi K.
dc.contributor.author Kraus, Tomáš
dc.contributor.author Meyer, Janis
dc.contributor.author Celikay, Kerem
dc.contributor.author Maiser, Andreas
dc.contributor.author Leonhardt, Heinrich
dc.contributor.author Rohr, Karl
dc.contributor.author Hocek, Michal
dc.contributor.author Cardoso, M. Cristina
dc.contributor.author Harz, Hartmann
dc.contributor.author Pradhan, Sunil Kumar
dc.contributor.author kuba, Miroslav
dc.date.accessioned 2025-01-23T17:54:48Z
dc.date.available 2025-01-23T17:54:48Z
dc.date.created 2025-01-10
dc.date.issued 2025-01-23
dc.description Chromatin dynamics play crucial roles in cellular differentiation, yet tools for studying global chromatin mobility in living cells remain limited. Here, we developed a new STED-compatible silicon rhodamine-linked SiR-dCTP nucleotide combined with SNTT1 to label chromatin in live cells and track chromatin mobility during neural differentiation. Using correlative microscopy, we quantified the labeled chromatin domain sizes using STED super resolution and confocal and we have labeled domains of the sizes closer to chromatin loop domains. Additionally, using this approach we demonstrate that chromatin mobility progressively decreases during the transition from human induced pluripotent stem cells (iPSCs) to neural stem cells (NSCs) and ultimately to neurons. This reduction in mobility correlates with differentiation state, suggesting a potential role for chromatin dynamics in cellular plasticity. Mechanistic insights into this phenomenon using global MNase assay reveals that the accessibility of chromatin decreases during neuronal differentiation suggesting that chromatin gets more restricted with implications for understanding chromatin regulation during development. de_DE
dc.identifier.uri https://tudatalib.ulb.tu-darmstadt.de/handle/tudatalib/4441
dc.language.iso en de_DE
dc.rights.licenseCC-BY-NC-4.0 (https://creativecommons.org/licenses/by-nc/4.0)
dc.subject SIR-dCTP de_DE
dc.subject SNTT1 de_DE
dc.subject chromatin mobility de_DE
dc.subject neural differentiation de_DE
dc.subject STED de_DE
dc.subject.classification 2.11-03
dc.subject.ddc 570
dc.title Superresolution-Compatible DNA Labeling Technique with Silicon Rhodamine -Linked Nucleotide Reveals Chromatin Mobility and Organization Changes During Neuronal Differentiation de_DE
dc.type Text de_DE
dc.type Software de_DE
dc.type Image de_DE
dcterms.accessRights restrictedAccess
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
tuda.project DFG | CA198/20-1 | Regulation der Säuge
tuda.project DFG | SFB1361,TP06 | TP_06_Cardoso_Mainz
tuda.unit TUDa

Files

Original bundle

Now showing 1 - 20 of 102
NameDescriptionSizeFormat
Sirdctp_chromatinmobility_datasets.z044 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z054 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z014 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z024 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z034 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z084 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z094 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z104 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z064 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z074 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z144 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z154 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z114 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z124 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z134 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z184 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z194 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z204 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z164 GBUnknown data format Download
Sirdctp_chromatinmobility_datasets.z174 GBUnknown data format Download
Simple item page

Collections