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Developmental changes in genome replication progression in pluripotent versus differentiated human cells
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2024-01-25
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DNA replication is a fundamental process ensuring the complete and accurate maintenance of the genome each time cells divide. This is particularly relevant early in development when cells divide profusely, later giving rise to entire organs. Increasing number of studies analyze DNA replication progression genome-wide but lack 3D spatial information as well as have a low temporal resolution. Importantly, there is little information regarding repeat elements which constitute more than half of the human genome. Here, we analyze and compare the genome replication progression in human stem cells, pluripotent stem cells and differentiated somatic cells. Using single-cell microscopic approaches and quantitative image analysis, we map the spatio-temporal genome replication of chromatin as a function of its compaction level. Furthermore, we mapped the replication timing of subchromosomal tandem repeat regions as well as the interspersed repeat sequences elements. Here, we found developmental changes in the replication timing of rDNA repeats. Finally, we compared the single-cell microscopic data with data from genome-wide sequencing approaches.
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Except where otherwise noted, this license is described as CC-BY-NC 4.0 - Attribution-NonCommercial 4.0 International